Bootheel Conference
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Campbell | Camels | Black/Yellow | Campbell | Dunklin | Public |
| Caruthersville | Tigers | Red/Yellow | Caruthersville | Pemiscot | Public |
| Hayti | Indians | Black/Yellow | Hayti | Pemiscot | Public |
| Kennett | Indians | Black/Yellow | Kennett | Dunklin | Public |
| Malden | Green Wave | Green/Yellow | Malden | Dunklin | Public |
| Portageville | Bulldogs | Blue/White | Portageville | New Madrid | Public |
| Senath-Hornersville | Lions | Black/Yellow | Senath | Dunklin | Public |
| South Pemiscot | Bulldogs | Black/Yellow | Steele | Pemiscot | Public |
Jefferson County Athletic Association
| School Name | Mascot | Colors | City | County | School Type | Division |
|---|---|---|---|---|---|---|
| Crystal City | Hornets | Black/Red | Crystal City | Jefferson | Public | Small Schools |
| DeSoto | Dragons | Green/Yellow | DeSoto | Jefferson | Public | Large Schools |
| Festus | Tigers | Black/Yellow | Festus | Jefferson | Public | Large Schools |
| Grandview | Eagles | Black/Yellow | Hillsboro | Jefferson | Public | Small Schools |
| Herculaneum | Blackcats | Black/Red | Herculaneum | Jefferson | Public | Small Schools |
| Hillsboro | Hawks | Blue/Yellow | Hillsboro | Jefferson | Public | Large Schools |
| Jefferson | Blue Jays | Blue/White | Festus | Jefferson | Public | Small Schools |
| Perryville | Pirates | Green/Yellow | Perryville | Perry | Public | Large Schools |
| St. Vincent | Indians | Blue/White | Perryville | Perry | Private | Small Schools |
| Windsor | Owls | Yellow/Maroon | Imperial | Jefferson | Public | Large Schools |
Mineral Area Activities Association (MAAA)
| School Name | Mascot | Colors | City | County | School Type | Division |
|---|---|---|---|---|---|---|
| Arcadia Valley | Tigers | Black/Yellow | Ironton | Iron | Public | Small Schools |
| Bismarck | Indians | Red/Yellow | Bismarck | St. Francois | Public | Small Schools |
| Central | Rebels | Red/Blue | Park Hills | St. Francois | Public | Large Schools |
| Farmington | Knights | Black/Yellow | Farmington | St. Francois | Public | Large Schools |
| Fredericktown | Blackcats | Black/Yellow | Fredericktown | Madison | Public | Small Schools |
| Kingston | Cougars | Green/Yellow | Cadet | Washington | Public | Small Schools |
| North County | Raiders | Blue/Yellow | Bonne Terre | St. Francois | Public | Large Schools |
| Potosi | Trojans | Purple/Yellow | Potosi | Washington | Public | Large Schools |
| Ste. Genevieve | Dragons | Green/Yellow | Ste. Genevieve | Ste. Genevieve | Public | Large Schools |
| Valle Catholic | Warriors | Blue/White | Ste. Genevieve | Ste. Genevieve | Private | Small Schools |
| Valley | Vikings | Black/Yellow | Caledonia | Washington | Public | Small Schools |
| West County | Bulldogs | Purple/Yellow | Leadwood | St. Francois | Public | Small Schools |
Mississippi Valley Conference
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Delta | Bobcats | Blue/White | Delta | Cape Girardeau | Public |
| Leopold | Wildcats | Blue/Yellow | Leopold | Bollinger | Public |
| Marquand-Zion | Tigers | Purple/Yellow | Marquand | Madison | Public |
| Meadow Heights | Panthers | Red/Yellow | Patton | Bollinger | Public |
| Zalma | Bulldogs | Yellow/Black | Zalma | Bollinger | Public |
| Greenville | Bears | Red/Yellow | Greenville | Wayne | Public |
Ozarks Foothills Conference
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Clearwater | Tigers | Yellow/Black | Piedmont | Wayne | Public |
| Doniphan | Dons | Yellow/Black | Doniphan | Ripley | Public |
| East Carter County | Redbirds | Red/Yellow | Ellsinore | Carter | Public |
| Greenville | Bears | Red/Yellow | Greenville | Wayne | Public |
| Naylor | Eagles | Black/Yellow | Naylor | Ripley | Public |
| Neelyville | Tigers | Yellow/Maroon | Neelyville | Butler | Public |
| Twin Rivers | Royals | Blue/White | Broseley | Butler | Public |
Scott-Mississippi Conference
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Chaffee | Red Devils | Black/Red | Chaffee | Scott | Public |
| Delta | Bobcats | Blue/White | Delta | Cape Girardeau | Public |
| East Prairie | Eagles | Black/Red | East Prairie | Mississippi | Public |
| Oak Ridge | Blue Jays | Blue/White | Oak Ridge | Cape Girardeau | Public |
| Oran | Eagles | Blue/Yellow | Oran | Scott | Public |
| Scott City | Rams | Blue/Yellow | Scott City | Scott | Public |
| Scott County Central | Braves | Black/Yellow | Sikeston | Scott | Public |
| Thomas W. Kelly | Hawks | Yellow/Maroon | Benton | Scott | Public |
Southeast Missouri (SEMO) Conference
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Cape Central | Tigers | Yellow/Black | Cape Girardeau | Cape Girardeau | Public |
| Charleston | Blue Jays | Blue/White | Charleston | Mississippi | Public |
| Dexter | Bearcats | Black/Red | Dexter | Stoddard | Public |
| Jackson | Indians | Black/Red | Jackson | Cape Girardeau | Public |
| Kennett | Indians | Black/Yellow | Kennett | Dunklin | Public |
| New Madrid County Central | Eagles | Green/Yellow | New Madrid | New Madrid | Public |
| Notre Dame | Bulldogs | Blue/White | Cape Girardeau | Cape Girardeau | Private |
| Poplar Bluff | Mules | Black/Maroon | Poplar Bluff | Butler | Public |
| Sikeston | Bulldogs | Black/Red | Sikeston | Scott | Public |
Stoddard County Activities Association (SCAA)
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Advance | Hornets | Black/Yellow | Advance | Stoddard | Public |
| Bell City | Cubs | Black/Red | Bell City | Stoddard | Public |
| Bernie | Mules | Blue/White | Bernie | Stoddard | Public |
| Bloomfield | Wildcats | Purple/Yellow | Bloomfield | Stoddard | Public |
| Dexter | Bearcats | Black/Red | Dexter | Stoddard | Public |
| Puxico | Indians | Yellow/Black | Puxico | Stoddard | Public |
| Richland | Rebels | Red/Gray | Essex | Stoddard | Public |
| Woodland | Cardinals | Red/Black | Marble Hill | Bollinger | Public |
Tri-County Conference (Southeast Division)
| School Name | Mascot | Colors | City | County | School Type |
|---|---|---|---|---|---|
| Clarkton | Reindeer | Purple/White | Clarkton | Dunklin | Public |
| Cooter | Wildcats | Red/White | Cooter | Pemiscot | Public |
| Delta C7 | Chargers | Blue/White | Deering | Pemiscot | Public |
| Gideon | Bulldogs | Black/Yellow | Gideon | New Madrid | Public |
| Holcomb | Hornets | Purple/Yellow | Holcomb | Dunklin | Public |
| North Pemiscot | Mustangs | Red/White | Wardell | Pemiscot | Public |
| Risco | Tigers | Yellow/Black | Risco | New Madrid | Public |
| Southland | Rebels | Red/White | Cardwell | Dunklin | Public |